I realize that it might be helpful to explain how to actually dial in a CO2 setup!
First, you have to keep as many variables constant as possible. If you are constantly changing these, it makes it VERY hard to dial in CO2 injection:
Things that change CO2 levels in the water are:
- Injection rate (bubbles per second for bubble counters, or SCCM if using flowmeter. Also rotatable/adjustable horizontal reactors)
- Temperature (warmer = less CO2, colder = more CO2)
- Surface agitation (how near your filter/lily pipe is towards the surface of the water)
- Surface scum (more scum=more CO2. Skimmers help remove scum)
- Time CO2 injection started (injecting earlier allows more time to reach CO2 equilibrium)
Here's a real example of how I get an aquarium CO2 dialed in with only drop checkers and the Hanna CO2 test kit
This is WAY easier to do without fish/shrimp/snails, since there's no danger if you "over-inject" CO2.
1) I get
everything set up. Tank, hardscape, light, filter, etc. I set my inline heater to a constant 74F. I lock my lily pipes in place, and check that I like the flow and surface agitation, and my skimmer is set up properly. I get a drop checker filled and installed. Once these things are how I like,
I keep them there and don't **** with them. This is also easier to get setup without plants, so you don't have to worry about unhappy plants while dialing in the setup.
2) I start CO2 injection in the evening at a level I think might be appropriate. I let it inject CO2 all night. I don't worry or care about wasting CO2. I try to inject for at least 8-10 hours or more.
3) In the morning of Day 1, I check the drop checker and take a CO2 reading with the Hanna test kit.
By injecting CO2 for ~10 hours, I can be sure I've reached whatever equilibrium level this current CO2/min injection setup is going to hit. If I like what I see (around 30-35ppm CO2, usually), I don't change a anything. I now set up my CO2 solenoid timer to come on anywhere from 3-5 hours before the lights normally would, and turn the CO2 off for the remainder of this day.
This step is used to determine what the actual ppm the injection/offgassing equilibrium reaches with my setup.
If I'm injecting too much CO2, I reduce the CO2/min rate on my needle valve or rotate my horizontal reactor (if running in overflow mode), and try again in 8-10 hours. Same for if I'm injecting too little.
4) Once I get the equilibrium-over-time level to the ppm CO2 I want, it's time to dial in the timer setup. I let the CO2 timer turn the solenoid on for the first time automatically in the early morning. At 9am (aka whenever I want lights to come on) I take a Hanna CO2 reading and check the DC. If the reading is within 5-7ppm of my targeted 30-35ppm CO2, I'm probably injecting early enough. If it's only at 20 or 25ppm CO2, and I haven't changed anything, then I know I will need to start CO2 injection earlier in the day.
This stem is used to determine AT WHAT TIME IN THE MORNING I need to start the previously-determined CO2 injection rate to reach near my desired 30-35ppm CO2 injection for the entire duration of the photoperiod.
5) If I like what I see, I let the CO2 inject normally as I would for the photoperiod. At 4pm, I take a Hanna CO2 reading and check my DC too.
If the reading at 4pm (or whenever you want CO2 to turn off) is at ~35ppm CO2, I've nailed it! If it's at 40 or 45+ ppm CO2, I'm injecting too much CO2/min.
6) For the next few days, I take a CO2 test reading at 9am (lights on) and at 4pm (CO2 off). If my CO2 level is ~30ppm at lights on, and ~35ppm at CO2 off, this setup is perfect.
And I'm done! I never have to worry about it again, right? Unless... If I adjust anything in a major way, I will need to do the same to dial it in. For example:
- Installing a more powerful filter will increase the flow, which changes the surface agitation and therefore the offgassing rate. Might need to dial in my CO2 again.
- Bumped my lily pipe and I can't remember how high I had it set up before. Might need to dial in my CO2 again.
- Went from 68F unheated room temp to an 86F discus tank. Might need to dial in my CO2 again.
- My CO2 cylinder ran out of CO2, and I had to install a new one which involved closing my regulator dials. Might need to dial in my CO2 again.
The easiest way to dial in your CO2 with a Hanna test kit is to simply start injection earlier in the morning than normal, and take a reading after 4-6 hours. The longer you give your CO2 time to inject, the more certain of the equilibrium level you can be. I usually find that anywhere from 4-6 hours is the minimum to get within 90% of the equilibrium rate, though in some setups it might take longer.
If your actual equilibrium level is 30-35ppm for 90% of the photoperiod, you will experience the best growth you've ever seen with your plants.