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New Yugang Testing

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Yeah, I'm definitely not using a diffuser. After some additional observation, I do think this is a case of air bubbles getting smashed into micro bubbles and passing right though the reactor. Surprising that it can appear as small as diffused CO2 mist but it's the most reasonable explanation. Made some adjustments to the skimmer and wasn't as noticeable today.

It certainly seems to fit the evidence. I'm glad you seem to have improved things.
 
I realize that it might be helpful to explain how to actually dial in a CO2 setup!

First, you have to keep as many variables constant as possible. If you are constantly changing these, it makes it VERY hard to dial in CO2 injection:

Things that change CO2 levels in the water are:
  • Injection rate (bubbles per second for bubble counters, or SCCM if using flowmeter. Also rotatable/adjustable horizontal reactors)
  • Temperature (warmer = less CO2, colder = more CO2)
  • Surface agitation (how near your filter/lily pipe is towards the surface of the water)
  • Surface scum (more scum=more CO2. Skimmers help remove scum)
  • Time CO2 injection started (injecting earlier allows more time to reach CO2 equilibrium)

Here's a real example of how I get an aquarium CO2 dialed in with only drop checkers and the Hanna CO2 test kit​


This is WAY easier to do without fish/shrimp/snails, since there's no danger if you "over-inject" CO2.

1) I get everything set up. Tank, hardscape, light, filter, etc. I set my inline heater to a constant 74F. I lock my lily pipes in place, and check that I like the flow and surface agitation, and my skimmer is set up properly. I get a drop checker filled and installed. Once these things are how I like, I keep them there and don't **** with them. This is also easier to get setup without plants, so you don't have to worry about unhappy plants while dialing in the setup.

2) I start CO2 injection in the evening at a level I think might be appropriate. I let it inject CO2 all night. I don't worry or care about wasting CO2. I try to inject for at least 8-10 hours or more.

3) In the morning of Day 1, I check the drop checker and take a CO2 reading with the Hanna test kit. By injecting CO2 for ~10 hours, I can be sure I've reached whatever equilibrium level this current CO2/min injection setup is going to hit. If I like what I see (around 30-35ppm CO2, usually), I don't change a anything. I now set up my CO2 solenoid timer to come on anywhere from 3-5 hours before the lights normally would, and turn the CO2 off for the remainder of this day. This step is used to determine what the actual ppm the injection/offgassing equilibrium reaches with my setup.
If I'm injecting too much CO2, I reduce the CO2/min rate on my needle valve or rotate my horizontal reactor (if running in overflow mode), and try again in 8-10 hours. Same for if I'm injecting too little.

4) Once I get the equilibrium-over-time level to the ppm CO2 I want, it's time to dial in the timer setup. I let the CO2 timer turn the solenoid on for the first time automatically in the early morning. At 9am (aka whenever I want lights to come on) I take a Hanna CO2 reading and check the DC. If the reading is within 5-7ppm of my targeted 30-35ppm CO2, I'm probably injecting early enough. If it's only at 20 or 25ppm CO2, and I haven't changed anything, then I know I will need to start CO2 injection earlier in the day. This stem is used to determine AT WHAT TIME IN THE MORNING I need to start the previously-determined CO2 injection rate to reach near my desired 30-35ppm CO2 injection for the entire duration of the photoperiod.

5) If I like what I see, I let the CO2 inject normally as I would for the photoperiod. At 4pm, I take a Hanna CO2 reading and check my DC too.
If the reading at 4pm (or whenever you want CO2 to turn off) is at ~35ppm CO2, I've nailed it! If it's at 40 or 45+ ppm CO2, I'm injecting too much CO2/min.

6) For the next few days, I take a CO2 test reading at 9am (lights on) and at 4pm (CO2 off). If my CO2 level is ~30ppm at lights on, and ~35ppm at CO2 off, this setup is perfect.

And I'm done! I never have to worry about it again, right? Unless... If I adjust anything in a major way, I will need to do the same to dial it in. For example:

  • Installing a more powerful filter will increase the flow, which changes the surface agitation and therefore the offgassing rate. Might need to dial in my CO2 again.
  • Bumped my lily pipe and I can't remember how high I had it set up before. Might need to dial in my CO2 again.
  • Went from 68F unheated room temp to an 86F discus tank. Might need to dial in my CO2 again.
  • My CO2 cylinder ran out of CO2, and I had to install a new one which involved closing my regulator dials. Might need to dial in my CO2 again.
The easiest way to dial in your CO2 with a Hanna test kit is to simply start injection earlier in the morning than normal, and take a reading after 4-6 hours. The longer you give your CO2 time to inject, the more certain of the equilibrium level you can be. I usually find that anywhere from 4-6 hours is the minimum to get within 90% of the equilibrium rate, though in some setups it might take longer.

If your actual equilibrium level is 30-35ppm for 90% of the photoperiod, you will experience the best growth you've ever seen with your plants.

Regarding switching CO2 cylinders, is closing the regulator dial mandatory during the switch? I assume it can damage the regulator if the dial is not turned off? That is quite a bummer tbh, to have to fiddle with the needle valve for 1-2 days after each cylinder switch.
 
Obviously, I can crank up the injection rate, but I expected my injection rate to actually be reduced from what I'm used to, not more.
Given that the horizontal reactor is at low pressure (the pressure is at,ospheric plus the weight of the water column, well under 1 psi above ambient air pressure) whereas a ceramic diffuser the has significant pressure behind it, the bubbles in the bubble counter are at significantly lower pressure. A bubble under significant pressure is the same size as one that forms at higher pressure, but the amount of co2 molecules will be vastly different. In essence not all bubbles have the same amount of co2 in it so you really cant compare the bubble counter between the two.

I use my pressure knob as my fine adjust,ent screw. I initially turn it to full oressure and fill the reacotr chamber with the needle valve until I get overflow. I then back it off until overflow is down to every 5 seconds or so and then leave it at that level for about 10 minutes. Then slightly close the needle valve to get to where overflow is every 30 seconds or so, allowing a good 20 minutes or so between adjust,ents…. Once I get to that point I switch to the regulator pressure knob, Lowering it by 5 psi at a time. And checking on results after half an hour. I continue dropping pressure until I get to a puge of gas every minute to minute and a half.

Let the tank rin like this for at keast 24 hours.

The next day, check ph right before the co2 comes on. Retest every hour until ph stops dropping. Now you know how many hours it takes for ph to stop dropping after co2 comes on and reaches equilibrium…

Thats how long the co2 should be turned on before lights on. Adjust your solenoid timer accordingly.
 
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Given that the horizontal reactor is at low pressure (the pressure is at,ospheric plus the weight of the water column, well under 1 psi above ambient air pressure) whereas a ceramic diffuser the has significant pressure behind it, the bubbles in the bubble counter are at significantly lower pressure. A bubble under significant pressure is the same size as one that forms at higher pressure, but the amount of co2 molecules will be vastly different. In essence not all bubbles have the same amount of co2 in it so you really cant compare the bubble counter between the two.

I had honestly not considered this before but of course its basic science really. Genius !
 
Regarding switching CO2 cylinders, is closing the regulator dial mandatory during the switch? I assume it can damage the regulator if the dial is not turned off? That is quite a bummer tbh, to have to fiddle with the needle valve for 1-2 days after each cylinder switch.
You've got it right. This is one of the reasons why I love Flowmeters - they aren't just great for dialing in CO2, they also allow for easy cylinder tank swaps!

Yes, you can (and will) damage your CO2 regulator if you don't close the main valve fully before removing and reinstalling a cylinder. Also, make sure once the cylinder is attached, you open the main valve SLOWLY, both on the cylinder and on the regulator. Then adjust your needle valve from there.

What's so amazing about using a flow meter set up a bubble counter, is that it measures recitative flow of a gas, in a repeatable manner.

For example, I know that on my experiment tank, with my current set up, I need about 15 SCCM on my flowmeter during CO2 injection.

When my cylinder runs out, and I have to put a new one, all of the dials will be a little bit different when I attach the new cylinder. Having to dial in a bubble counter with a crappy needle valve (looking at you, FZONE) all over again is horrible. Especially when it can take a day or two for it to finally settle in.

With a flow meter, I just attach the new cylinder, adjust the dials until I get 15 SCCM again, and I'm good to go.

The only downside to flow meters are that there is a minimum tank size for them, because flowmeters don't really work in the ultra low ranges that nano tanks require. If you have a small to medium size tank, chances are you are stuck with a bubble counter.

If your tank is 30 gallons or more, you could probably use a flow meter. If it's 50 to 60 gallons or more, you can definitely use a flow meter.
 
Yes, you can (and will) damage your CO2 regulator if you don't close the main valve fully before removing and reinstalling a cylinder. Also, make sure once the cylinder is attached, you open the main valve SLOWLY, both on the cylinder and on the regulator.
Also not a bad practice to crack open the main valve to blow out any dust or debris that could be in the threaded outlet before attaching your regulator to the tank.
 
You've got it right. This is one of the reasons why I love Flowmeters - they aren't just great for dialing in CO2, they also allow for easy cylinder tank swaps!

Yes, you can (and will) damage your CO2 regulator if you don't close the main valve fully before removing and reinstalling a cylinder. Also, make sure once the cylinder is attached, you open the main valve SLOWLY, both on the cylinder and on the regulator. Then adjust your needle valve from there.

What's so amazing about using a flow meter set up a bubble counter, is that it measures recitative flow of a gas, in a repeatable manner.

For example, I know that on my experiment tank, with my current set up, I need about 15 SCCM on my flowmeter during CO2 injection.

When my cylinder runs out, and I have to put a new one, all of the dials will be a little bit different when I attach the new cylinder. Having to dial in a bubble counter with a crappy needle valve (looking at you, FZONE) all over again is horrible. Especially when it can take a day or two for it to finally settle in.

With a flow meter, I just attach the new cylinder, adjust the dials until I get 15 SCCM again, and I'm good to go.

The only downside to flow meters are that there is a minimum tank size for them, because flowmeters don't really work in the ultra low ranges that nano tanks require. If you have a small to medium size tank, chances are you are stuck with a bubble counter.

If your tank is 30 gallons or more, you could probably use a flow meter. If it's 50 to 60 gallons or more, you can definitely use a flow meter.
thanks for clarification.
what kind of flow meter are you refering to? didnt hear about that before...
 
I recommend a Dwyer 151-RMA, the 5-50CC/min model. Price point is just right for what you get, IMO.

You don't need the model with a valve if you can just use your regulator's needle valve.
Someone mentioned purchasing and testing a less expensive lower range flow meter off AliExpress but I don't remember what thread that was in. Curious how that worked out, if it's been tested yet.
 
I recommend a Dwyer 151-RMA, the 5-50CC/min model. Price point is just right for what you get, IMO.

You don't need the model with a valve if you can just use your regulator's needle valve.

Question - WHich connectors do you like to use to connect standard 6/4mm CO2 line ?
 
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