As far as my tank goes, zinc has caused me the most troubles. Years ago I saw immediate tox symptoms after routine Flourish use, dosing per the instructions. Flourish has a lot of zinc compared to other micros. More recently I kept zinc, boron and copper in individual bottles just to see if I could isolate the problems, and it seemed zinc doses never fixed anything, and the tank did better without it (with SafeTSorb substrate). I also transitioned to a low-tech "kill tank" this year (no CO2, no ferts, lower lighting) and some dormant AR Mini came back to life. Even with 100% RO water, I can never seem to dose the levels of zinc that other people manage to do.